Gain years of experience
in just a day.

SERVICES

Training – On site lab-training > PGS2: embryo and sperm chromosomal analysis

Objective:
The Main goal of this part of the training program is to provide the trainees with the ability to perform sperm and blastomere fluorescent in situ hybridization in order to provide information about sperm aneuploidies preimplantational genetic screening procedures (PGS).

Facilities and equipments:
PGS labs, equipped with fluorescent microscopy, laminar flow hoods, hybridization ovens, centrifuges, thermal baths, etc…

Procedures to be learnt:
Itempart of the training program is to provide the trainees with the ability to perform sperm and blastomere fluorescent in situ hybridization in order to provide information about sperm aneuploidies preimplantational genetic screening procedures (PGS). ized list of procedures that the attendee will be able to fully conduct when finished.

Duration ranges:
Recommended training time ranges depending on the student previous background and expertise, as recommended by the experts committee.

Capacity:
Definition of the maximum number of affordable trainees per year. The number of trainees per year will be significantly lower, since it is a centralized program for all IVI group. In this sense, 2-3 trainees per year is the most appropriate number, being able to consider an increase, if requested, increasing the number of professionals.

Include:
Assigned tutors. Certificate of attendance and accomplishment, issued by IVI Docencia, from Instituto Universitario IVI, Universitat de València.

Standard operation procedures

  • Safety guidelines for the PGS-chromosomes laboratory
  • Reports preparation
  • PGS-chromosomes equipment cleaning and maintenance
  • Good practices in the PGS-chromosomes lab
  • Quality controls
  • Culture media preparation
  • Laboratory setup
  • Sperm FISH
  • Basic sperm analysis
  • Sperm morphology
  • Solution preparation
  • Sperm fixation for FISH
  • fluorescent in situ hybridization procedures for sperm cells
  • Signals count and interpretation
  • Statistical analysis of the results
  • Stocks control
  • PGS blastomere FISH
  • Nucleii identification and location
  • Blastomere FISH protocols
  • Signals count and interpretation
  • Stocks control